tsa vividtm fluorophore kit 650 (Tocris)

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Tocris tsa vividtm fluorophore kit 650
Tsa Vividtm Fluorophore Kit 650, supplied by Tocris, used in various techniques. Bioz Stars score: 94/100, based on 27 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/tsa+vivid+fluorophore+650+7527/TSA+Vivid+Fluorophore+kit+650/pmc13015052-93-45-50
Average 94 stars, based on 27 article reviews

tsa vividtm fluorophore kit 650 - by Bioz Stars, 2026-09

94/100 stars

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RNAscope:

Article Title: Mechanobiological Specialization of Choroid Plexus Macrophages Defined by Titin Expression
Article Snippet: For titin immunofluorescence, primary antibody incubation with rabbit anti-titin (Proteintech Catalog #27867-1-AP) at 1:250 dilution and mouse anti-CD68 antibody (Millipore Cat.#168M-95) at 1:250 was conducted overnight at 4°C, followed by detection with secondary donkey anti-rabbit Alexa Fluor 488 (Thermo Fisher Scientific, Cat. #A21206) (1:500) and goat anti-mouse Alexa Fluor 647 (Thermo Fisher Scientific, Cat. #A32728), with nuclei counterstaining with DAPI. .. RNA probes were obtained from ACDBio, Inc., Newark, CA, for TTN (RNAscope Probe Hs-TTN, Cat. #550361) and detected using TSA Vivid Fluorophore Kit 650 (Tocris Bioscience, Cat. #7527). ..

other:

Article Title: Distinct roles for MNK1 and MNK2 in social and cognitive behavior through kinase-specific regulation of the synaptic proteome and phosphoproteome.
Article Snippet: Hybridization of probes and subsequent signal amplification were carried out using hydrogen peroxide and protease III, followed by the reagents in the Multiplex Fluorescent Detection Reagents v2 kits (Advanced Cell Diagnostics (ACD); Hayward, CA) as described in the manufacturer’s protocol.

Article Title: Endothelial USP8 is essential for angiogenesis
Article Snippet: Commercially available RNAscope probes for Acta2 (Catalog # 319531-C2), USP8 (Catalog # 1305711-C1) and Pecam (Catalog # 316721-C3) were acquired from Biotechne.

Article Title: Endothelial USP8 is essential for angiogenesis.
Article Snippet: Commercially available RNAscope probes for Acta2 (Catalog # 319531-C2), USP8 (Catalog # 1305711-C1) and Pecam (Catalog # 316721-C3) were acquired from Biotechne.

Amplification:

Article Title: Intranasal SARS-CoV-2 infection changes the transcriptome of the mouse trigeminal ganglion and brainstem: potential mechanisms underlying headache and trigeminal pain presentation in COVID-19
Article Snippet: Peroxidases and proteins were removed sequentially by RNAscope H 2 O 2 and protease reagents (Advanced Cell Diagnostics, PN 322381) before hybridizing the mRNAs in the sections with SARS-CoV-2 probes: RNAscopeTM Probe-V-nCoV-orf1ab-O1 (Advanced Cell Diagnostics, Cat. #859981), RNAscopeTM Probe-V-nCoV-N (Advanced Cell Diagnostics, Cat. #846081-C2), RNAscopeTM Probe-V-nCoV2019-S (Advanced Cell Diagnostics, Cat. #848561-C3), and RNAscopeTM Probe-Mm-Trpv1 (Advanced Cell Diagnostics, Cat. #313331). .. Next, probes were amplified with HRP recombinant molecules by RNAscopeTM Multiplex Fluorescent Detection Kit v2 (Advanced Cell Diagnostics, Cat. #323110) before reacting with the following dyes: TSA VividTM Fluorophore Kit 520 (Tocris Bioscience, Cat. #7523), TSA VividTM Fluorophore Kit 570 (Tocris Bioscience, Cat. #7526), and TSA VividTM Fluorophore Kit 650 (Tocris Bioscience, Cat.#7527). .. Each experiment was validated by using the RNAscopeTM 3-plex Positive Control Probe-Mm (Advanced Cell Diagnostics, Cat. #320881) and RNAscopeTM Probe Diluent (negative control) (Advanced Cell Diagnostics, Cat. #300041) (not shown).

Article Title: Intranasal SARS-CoV-2 infection changes the transcriptome of the mouse trigeminal ganglion and brainstem: potential mechanisms underlying headache and trigeminal pain presentation in COVID-19.
Article Snippet: Peroxidases and proteins were removed sequentially by RNAscope H2O2 and protease reagents (Advanced Cell Diagnostics, PN 322381) before hybridizing the mRNAs in the sections with SARS-CoV-2 probes: RNAscopeTM Probe-V-nCoV-orf1ab-O1 (Advanced Cell Diagnostics, Cat. #859981), RNAscopeTM Probe-V-nCoV-N (Advanced Cell Diagnostics, Cat. #846081-C2), RNAscopeTM Probe-V-nCoV2019-S (Advanced Cell Diagnostics, Cat. #848561-C3), and RNAscopeTM Probe-Mm-Trpv1 (Advanced Cell Diagnostics, Cat. #313331). .. Next, probes were amplified with HRP recombinant molecules by RNAscopeTM Multiplex Fluorescent Detection Kit v2 (Advanced Cell Diagnostics, Cat. #323110) before reacting with the following dyes: TSA VividTM Fluorophore Kit 520 (Tocris Bioscience, Cat. #7523), TSA VividTM Fluorophore AR TIC LE IN PR ES S Kit 570 (Tocris Bioscience, Cat. #7526), and TSA VividTM Fluorophore Kit 650 (Tocris Bioscience, Cat.#7527). .. RNAscope fluorescence in situ hybridization (FISH) in lung and TG Sections were washed in 0.1 M PBS, then heated for antigen retrieval for 5 min in +100 °C RNAscope Target Retrieval Reagents (Advanced Cell Diagnostics, Cat. #322000).

Article Title: Enamel decussation pattern originates from directional sliding of ameloblasts
Article Snippet: RNAscopeTM Multiplex Fluorescent v2 Assay (323100, Advanced Cell Diagnostics, USA) was used for in situ hybridization according to the manufacturer’s manual with the following probes: Sox2 (401041-C2, Advanced Cell Diagnostics, USA) and Sox10 (435931, Advanced Cell Diagnostics, USA). .. TSA VividTM Fluorophore Kit 570 (7526, Tocris Bioscience, UK) and TSA VividTM Fluorophore Kit 650 (7527, Tocris Bioscience, UK) were used for signal amplification. ..

Recombinant:

Article Title: Intranasal SARS-CoV-2 infection changes the transcriptome of the mouse trigeminal ganglion and brainstem: potential mechanisms underlying headache and trigeminal pain presentation in COVID-19.
Article Snippet: Peroxidases and proteins were removed sequentially by RNAscope H2O2 and protease reagents (Advanced Cell Diagnostics, PN 322381) before hybridizing the mRNAs in the sections with SARS-CoV-2 probes: RNAscopeTM Probe-V-nCoV-orf1ab-O1 (Advanced Cell Diagnostics, Cat. #859981), RNAscopeTM Probe-V-nCoV-N (Advanced Cell Diagnostics, Cat. #846081-C2), RNAscopeTM Probe-V-nCoV2019-S (Advanced Cell Diagnostics, Cat. #848561-C3), and RNAscopeTM Probe-Mm-Trpv1 (Advanced Cell Diagnostics, Cat. #313331). .. Next, probes were amplified with HRP recombinant molecules by RNAscopeTM Multiplex Fluorescent Detection Kit v2 (Advanced Cell Diagnostics, Cat. #323110) before reacting with the following dyes: TSA VividTM Fluorophore Kit 520 (Tocris Bioscience, Cat. #7523), TSA VividTM Fluorophore AR TIC LE IN PR ES S Kit 570 (Tocris Bioscience, Cat. #7526), and TSA VividTM Fluorophore Kit 650 (Tocris Bioscience, Cat.#7527). .. RNAscope fluorescence in situ hybridization (FISH) in lung and TG Sections were washed in 0.1 M PBS, then heated for antigen retrieval for 5 min in +100 °C RNAscope Target Retrieval Reagents (Advanced Cell Diagnostics, Cat. #322000).

Multiplex Assay:

Article Title: Intranasal SARS-CoV-2 infection changes the transcriptome of the mouse trigeminal ganglion and brainstem: potential mechanisms underlying headache and trigeminal pain presentation in COVID-19.
Article Snippet: Peroxidases and proteins were removed sequentially by RNAscope H2O2 and protease reagents (Advanced Cell Diagnostics, PN 322381) before hybridizing the mRNAs in the sections with SARS-CoV-2 probes: RNAscopeTM Probe-V-nCoV-orf1ab-O1 (Advanced Cell Diagnostics, Cat. #859981), RNAscopeTM Probe-V-nCoV-N (Advanced Cell Diagnostics, Cat. #846081-C2), RNAscopeTM Probe-V-nCoV2019-S (Advanced Cell Diagnostics, Cat. #848561-C3), and RNAscopeTM Probe-Mm-Trpv1 (Advanced Cell Diagnostics, Cat. #313331). .. Next, probes were amplified with HRP recombinant molecules by RNAscopeTM Multiplex Fluorescent Detection Kit v2 (Advanced Cell Diagnostics, Cat. #323110) before reacting with the following dyes: TSA VividTM Fluorophore Kit 520 (Tocris Bioscience, Cat. #7523), TSA VividTM Fluorophore AR TIC LE IN PR ES S Kit 570 (Tocris Bioscience, Cat. #7526), and TSA VividTM Fluorophore Kit 650 (Tocris Bioscience, Cat.#7527). .. RNAscope fluorescence in situ hybridization (FISH) in lung and TG Sections were washed in 0.1 M PBS, then heated for antigen retrieval for 5 min in +100 °C RNAscope Target Retrieval Reagents (Advanced Cell Diagnostics, Cat. #322000).

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